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KMID : 1007520130220041113
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Food Science and Biotechnology
2013 Volume.22 No. 4 p.1113 ~ p.1120
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Anti-inflammatory Activity of Ethanolic Extract of Sargassum sagamianum in RAW 264.7 Cells
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Kim Min-Ji
Kim Koth-Bong-Woo-Ri
Jeong Da-Hyun
Ahn Dong-Hyun
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Abstract
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Aim of the study is to evaluate the antiinflammatory effects of ethanolic extract of the marine brown alga Sargassum sagamianum collected from Yeonhwari coast of Korea. Ethanolic extract of S. sagamianum (SA-E extract) inhibited expression of nitric oxide (NO) and cytokines (IL-6, IL-1β, and TNF-α) as well as inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)- induced RAW 264.7 cells without affecting cell viability. In addition, the expression of nuclear factor (NF)-κB p65 was suppressed by SA-E extract. Furthermore, the rate of formation of edema in the mouse ear was reduced by 46% at the highest dose tested (250 mg/kg) compared to that in the control. This study suggests that SA-E extract exerts potent inhibitory effects on LPS-induced expression of inflammatory mediators such as NO, iNOS, COX-2, and cytokines in macrophages through suppression of the NF- KB p65 pathway. SA-E extract might have potential clinical applications as an anti-inflammatory agent.
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KEYWORD
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Sargassum sagamianum, inflammation, cyclooxygenase-2, inducible nitric oxide synthase, nuclear factor-KB
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